Related Products:

Society for Amateur Scientists

Design Your Experiments: Part II, Noise

by Kevin Kilty

I'll spend at least three parts of this series on design of experiments, maybe more if I think it useful, discussing models and their characteristics.

Scientists can never actually say what something is, but only how it behaves; and models form the basis for describing how a thing behaves. The models I typically think about are mathematical, but models don't have to be so limited. They could be purely descriptive. What is essential about models is that they give us a means to describe, and predict behavior; and, provide a way to test theories. It is this last point, about being able to test theories, which separates scientific theories from the kind of theories, interpretations actually, that the literary critic speaks of.

First and most fundamental, we have to recognize that the measurements we make are not exact, but include the influence of error or noise. Many people refer to the inexactness of experiments as "experimental error." This is what I mean by the word noise, except I think that noise describes the issue better because some of the inexactness of experiments is unavoidable, and therefore unlike an error of some type.

Just as experimental results contain noise, our models have to reflect this reality as well. Noise is actually quite difficult to define--you've all no doubt heard the adage that one person's noise is another's signal. I'll define noise as any influence which prevents us from attaining identical measured values, or identical results, on repeated versions of an experiment. Noise may come from many sources. I'll give you three examples.

• First, some processes have inherent noisiness. Thermal noise, Johnson noise, is a good example of this. All noise of this type comes from the molecular and atomic world being fundamentally statistical.
• Second, we cannot make measurements that are absolutely identical from one try to the next because our measuring equipment has limited resolution and precision. This leads to slight uncertainty in measured results.
• Third, it is darned difficult to repeat any experiment under identical conditions. There are always unaccounted-for influences in an experiment which cause it to be unique, and the result of these unknown influences is noise.

One thing you probably notice from this short list is that it covers two completely different types of noise the sum of which become experimental noise. The first type affects the phenomenon itself. To measure this type of noise we must replicate experiments. We perform experiments on a limited area, or a limited amount of material, or on a group of plants or animals. These smallest units of stuff on which we perform experiments are experimental units. We replicate an experiment on various experimental units that we try to make as identical to one another as possible. Sometimes we can use the same experimental unit over again sometimes we cannot.

The second type of noise in our short list comes from our inability to measure anything with absolute accuracy. This is observational noise. To measure this we can repeat our measurement. The smallest unit of outcome that we measure in the aftermath of an experiment is the observational unit. The observational unit doesn't have to be identical to the experimental unit. It can be only a portion of the experimental unit. Observational error or noise is a part of the total noise in an experiment but it cannot tell us anything about the experimental noise. Only replications of the experiment will fully measure experimental noise.

To avoid being too abstract, let me provide an example. Suppose we wish to measure how affective is an antiseptic for sterilizing a surface. We take a single surface that we believe is uniform--for instance, a single surface used to prepare food. We subdivide this uniform surface into several smaller areas. Each of these is a possible experimental unit to which we can apply the antiseptic (test areas) or not (control areas). Since we intend each subdivision to be identical, we are eliminating possible sources of experimental noise.

To further reduce sources of noise, we need to assign each subdivision into the control group or the test group randomly. This helps to prevent either pure bad luck or subconcious decision from adding noise or error to the experiment. There are many ways to do this. For instance, we could subdivide the surface into a Latin Square-something I'll discuss in a further installment. Or, we could organize the surface into pairs of subdivided areas and use a coin flip to assign one half of the pair to the control or test. Making each experimental unit a pair is a well established technique for making experimental units that are nearly identical and for reducing experimental noise.

The experiment consists of applying antiseptic to the test half of each paired area, and also applying some type of placebo to the control half. The placebo is needed to keep the two halves of each experimental unit as identical as possible. Now it is time to measure the outcome of the experiment. The most reasonable way to do this is to count bacteria in each region. But it seems likely that our experimental units are too large to make a full census of bacteria. Instead, we will sample each area. These sampled areas are the observational units of this experiment, and it should be obvious that they are smaller than the experimental units. Even so, they are probably still large enough that we cannot count bacteria on them with complete accuracy. Nothing else about the experiment at this point affects its design.

Let me summarize this simple experimental design...

• Our experimental units consist of small areas of a surface that are uniform as possible, including being prepared in a uniform manner; and, which are divided into a test half and a control half. These are paired experimental units.
• The experiment consists of applying a treatment (antiseptic in this case) to the test half and applying some sort of placebo treatment to the control half of each unit.
• An experimental result is the difference in bacteria counts between the control and test halves of each experimental unit. Each such result is a replication of the basic experiment.
• Experimental noise consists of variation in the bacterial counts among the experimental units. Some of this variation comes from unaccounted-for variation in the surface and other factors. Some comes from measurement.
• Measurement of the experiment consists of counting bacteria in small sampled areas of each experimental unit. These samples are the observational units.
• The observational units are small, but still large enough to make a perfectly accurate count of bacteria impossible. We can repeat our count on each experimental unit to estimate observational noise.
• A portion of experimental noise is observational noise; the rest comes from unaccounted for influences.

Even though noise is a constant impediment to our search for truth, there is no reason to despair. We can handle noisy experiments if we learn to think statistically. Toward this goal in the next installment I'll present models of noise, and make recommendations on how to report noise, or uncertainty, in experimental results. Then we are ready to being looking at inference in the presence of noise and how this affects experimental design.

Reprinted from: